What principle does the new crown antigen detection kit show the result?

answer:

The kit uses immunity. Under the stimulation of exogenous protein, the organism produces an antibody that specifically identifies the exogenous protein. If the exogenous protein comes from the antibodies of other species, the antibody that recognizes the antibody can also be generated, which is usually the dichryomotic. In the recognition of the new coronary virus, one antibody can be a mouse source antibody that identifies the virus shell protein (injection of the virus shell into the mouse body, the antibody produced by the mouse). Rabbit source antibodies (after purifying the mouse source antibody, injection into the rabbit body, the antibody produced by the rabbit).

FIG1 one anti -two anti -identification mechanism

The antibody can use chemical reactions and specific labels to make it easy to identify. Pink colloidal gold or latex particles are usually used in the kit

Fig2 colloid gold has different colors in different sizes

Under normal circumstances, the virus recognizes the surface receptor of the cells, enters the cells, completes nucleic acid replication, shell protein expression, shell protein and nucleic acid assembly in the cells, and finally releases cells in the form of virus particles The same process is the same.

Fig3 virus amplification in cells

Scrape the nasal or oral cavity in the wipe, mainly epithelial cells, and a small amount of virus particles. We add the scraped swabs to the cracking solution, use high salt and downturn to destroy the cell membrane, and obtain the cell content. If the cells have been infected, there will be viral protein expression in the cell, which will be dissolved in it. In the solution.

The pattern diagram of the kit is shown in the figure. The bottom plate provides support, and the sample pad absorbs the cracking solution of drip adding in; the coupling cushion contains antiviral antibody antibody with colloidal gold or coloring latex marking; Antibodies with antiviral antibodies fixed with antiviral antibodies are the two antibodies mentioned before; water absorption pads are the absorption of the last excess liquid.

When you add the sample to the sample, the wet sample pad and the puppet pad, so that the antibody in the puppet pad to identify whether there is antigen in your solution. It is dissolved in the solution in the form of a single form. The solution will then analyze on the NC membrane (nitrate cellulose membrane).

Finally attach the effectiveness of nucleic acid detection and antigen detection comparison:

by a large naked ape

- END -