How trouble is the detection of nucleic acid?I'm sorry after reading

Recently everyone's daily life

is not it:

Do nucleic acid → go to work → go home → do nucleic acid

also

The most urgent thing everyone is

Reporting nucleic acid

Many people think

Nucleic acid detection is like blood routine

The result was found-

what is the problem?

Professionals make answers-

Real nucleic acid detection

Not as simple as you think!

Nuclear sampling is usually performed at the designated location.

Generally, the sampling point and the detection point are not together, and it is impossible to pick one, so it will be delayed here.

So some people say, can't you detect at the sampling point?

Nucleic acid detection is extremely sensitive and precise experiments. The nucleic acid detection laboratory has special environmental requirements, which can be detected without just opening a place.

It is also the reason why a special square laboratory can be established in order to perform nucleic acid testing.

Under the close packaging, the specimen was escorted to the laboratory and was signed by the laboratory to enter the information system.

This step requires one by one for disassembly packaging, and it is a double -layer sealed packaging, and without saying thousands of packaging, it can be disassembled for a day .. ...

Each one is sprayed to do a good job of biological safety protection.

With the sound of "drop", the information of the specimen will be entered into the system and connected to the health code.

During this period, all handmade is not much faster than sampling.

Especially in massive specimens, 0 errors must be guaranteed. You cannot Zhang Guan Li Dai, or the record information is recorded, and you have to find less or not. You have to find it again.

After all, it is related to everyone's health, and carelessness is absolutely not allowed.

Can I detect it directly when the specimen arrives at the laboratory?

It is also necessary to proceed before the specimen and extract the nucleic acid in the specimen to expand.

The question is here again:

If the nucleic acid extracted from the specimen is used as a piece of land, and the new crown virus is compared to peanuts, how do you know if there are peanuts in the many elements of the land?

Then the whole point of fertilizer and water, let peanuts grow, one change, two changes, four, four changes to eight ... This is easy to know.

In the same way, the virus is invisible, and the trace specimens are not easy to detect, so it is easy to make it more than to detect it.

In this process, the medical staff still had to unscrew the lid one by one and use a sample gun to draw the specimen for nucleic acid extraction —

Sit in front of the biological safety cabinet, unscrew thousands of lids, poke thousands of gun heads to draw thousands of specimens, and then kill thousands of guns, screw thousands of lids ....

For them, this step can be said to be very sour ~

This step is completely 0 pollution, strict and accurate operation,

There are as many reagents, how many EP test tubes,

The workload of thousands of guns ~

And all handmade packaging, it must be protected, it must not be polluted!

In case you fall down, you will get a wrong result:

There was no peanut in the soil, but a bunch of peanuts grew. After all, the power of the exponential increase was terrible.

This step is still all handmade, and the medical staff needs to focus on the whole process.

Make sure 0 mistakes!

And the extraction nucleic acid is easily polluted and easy to degrade, so the operation must be more cautious and cautious! After all, there are dozens of specimens in one board!

At the same time, the extraction nucleic acid only needs to add 5ul into the reagent system for amplification.

What is the concept of 5ul?

One -tenth of the water, you can only see the point on the head with a small gun head, and also require the operation process: fast! stable! allow!

This process takes 1-2 hours, and once the instrument starts the amplification process, it cannot stop adding a new specimen midway. After the results of this batch of results are completed, the next batch of specimens can be enlarged. Essence

Therefore, this is one of the reasons why nucleic acid detection cannot be performed at the time of testing, because as soon as the instrument is turned on, it cannot be stopped, and this batch must be run to perform the next batch.

If you really want to do it with you, then you need to say it like Akimid--

"Give me a long enough pole and a fulcrum, I can leverage the earth." Similarly, I can also say-

Give me enough people, enough instruments and venues, and I can do the new crown nucleic acid specimen to go to the test.

But is it enough to finish the above?

Definitely not!

When performing each test, you need to add a variety of negative controls. The positive control of quality control and physiological saline is used to monitor the quality of the experimental process to ensure the reliability and accuracy of the test results.

After all, there are too many steps in the experimental process, and there are too many potential interference factors, so you need to take such measures per batch to ensure the quality of the test.

After the results are come out, you need to check the results, check the specimen information, and release the results. Data transmission (that is, a green code).

Source: "Shenzhen Full Contact" public account

What are the most afraid of inspectors?

It is the failure of the expansion.

Although careful and careful, it is difficult to ensure that each result is so perfect.

Then, you know,

Looking back on the process, analyze the problems, find the steps that may be affected, and come again.

When running instrument

Can the testing staff be idle?

Thinking too beautiful!

When the instrument is running, the new batch of specimens come again, and the information will be recorded, the acidity is made, and the sample ...

Many preparations are interspersed in the middle:

The consumption of the gun head (2/specimen, thousands of specimens ≈1,000 gun heads, you calculate, only 96 gun heads), consumables preparation, instrument and equipment maintenance, environmental monitoring, massage cleaning, experimental experiments, experimental experiments The room ultraviolet, in addition, you have to go to the toilet within a day. Don't worry, for your green code

Everyone is "desperate"

However, despite the cumbersome process and many factors, please rest assured that medical staff have undergone strict theoretical operation training and assessment, and they are all holding a certificate.

Although it is difficult to do with measuring, the results are as fast as routine blood urine, but they are also rushing to work non -stop and strictly produce results within the prescribed time.

At present, all staff members have canceled their rest and fully support the detection of nucleic acids to shorten the results of the results. Your anxiety is also their hearts.

In this way, every day, everyone cooperates to ensure that the test results of a batch of nucleic acid tests are issued on time under the super logical arrangement.

Source: Yunnan Health Commission, Yunnan Communist Youth League

Edit: Pan Yi

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